Oligo™ Research Published in Esteemed Scientific Journal
Minimization of free radical damage by metal catalysis of multivitamin/ multimineral supplements
A B Rabovsky1, A M Komarov2, J Ivie1, and G.R. Buettner3
1Research & Technology Development, Melaleuca Inc.,
2Department of Biochemistry, GWU, 3The University of Iowa


Multivitamin/multimineral complexes are the most common dietary supplements. Besides quality ingredients and the amount of each ingredient in a product, bioavailability is a major concern. Unlike minerals in natural foods that are incorporated in bioorganic structures, minerals in dietary supplements are usually in an inorganic form: sulfates, chlorides, oxides etc. Unfortunately, the majority of minerals in these forms precipitate at the neutral pH of the small intestine, making absorption questionable. In addition, some minerals catalyze free radical generation, depending on their form. Thus, antioxidants in supplements could be oxidized during digestion. We have created a new complexing environment for minerals that consists of an amino acid chelate and non-digestible oligosaccharide (AAOS). All essential minerals in this form are soluble at intestinal pH. Even though soluble, the commonly used form of copper - gluconate - generates a flux of free radicals similar to the inorganic forms. Monitoring of ascorbate radical generated by different forms of copper shows that ascorbate is oxidized much more slowly with the AAOS matrix. Direct measurement of the oxidation of ascorbic acid (vitamin C) and gallic acid (a typical antioxidant ingredient derived from fruits) by different forms of minerals confirmed the ability of AAOS to slow these oxidations. Similar results were observed with iron-catalyzed formation of hydroxyl radicals (Fenton reaction), as measured by EPR spin trapping. In addition, the relative rates of oxidation of 2’,7’-dichlorodihydrofluorescein by H2O2 with copper were: sulfate > gluconate > glycinate > AAOS. When compared to traditional forms of minerals used in supplements, we conclude that the oxidative loss of antioxidants in solution at physiological pH is much slower when AAOS is used.
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1 A.B.Rabovsky, A.M.Komarov, J.Ivie, G.R.Buettner, "Minimization of free radical damage by metal catalysis of multivitamin/multimineral supplements," Free Radical Biology & Medicine, Vol. 45, Supp. 1, 2008, pg. S128.
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