|Oligo™ Research Published in Esteemed Scientific Journal
|Minimization of free radical damage by metal catalysis of multivitamin/ multimineral supplements
A B Rabovsky1, A M Komarov2, J Ivie1, and G.R. Buettner3
1Research & Technology Development, Melaleuca Inc.,
2Department of Biochemistry, GWU, 3The University of Iowa
Multivitamin/multimineral complexes are the most common
dietary supplements. Besides quality ingredients and the amount
of each ingredient in a product, bioavailability is a major concern.
Unlike minerals in natural foods that are incorporated in
bioorganic structures, minerals in dietary supplements are
usually in an inorganic form: sulfates, chlorides, oxides etc.
Unfortunately, the majority of minerals in these forms precipitate
at the neutral pH of the small intestine, making absorption questionable. In addition, some minerals catalyze free radical
generation, depending on their form. Thus, antioxidants in
supplements could be oxidized during digestion. We have
created a new complexing environment for minerals that
consists of an amino acid chelate and non-digestible
oligosaccharide (AAOS). All essential minerals in this form are
soluble at intestinal pH. Even though soluble, the commonly
used form of copper - gluconate - generates a flux of free
radicals similar to the inorganic forms. Monitoring of ascorbate
radical generated by different forms of copper shows that
ascorbate is oxidized much more slowly with the AAOS matrix.
Direct measurement of the oxidation of ascorbic acid (vitamin C)
and gallic acid (a typical antioxidant ingredient derived from
fruits) by different forms of minerals confirmed the ability of
AAOS to slow these oxidations. Similar results were observed
with iron-catalyzed formation of hydroxyl radicals (Fenton
reaction), as measured by EPR spin trapping. In addition, the
relative rates of oxidation of 2’,7’-dichlorodihydrofluorescein by
H2O2 with copper were: sulfate > gluconate > glycinate > AAOS.
When compared to traditional forms of minerals used in
supplements, we conclude that the oxidative loss of antioxidants
in solution at physiological pH is much slower when AAOS is
|1 A.B.Rabovsky, A.M.Komarov, J.Ivie, G.R.Buettner, "Minimization of free radical damage by metal catalysis of multivitamin/multimineral supplements," Free Radical Biology & Medicine, Vol. 45, Supp. 1, 2008, pg. S128.